Extension of “Interfacial Adsorption Denaturation” Behavior Interpretation Based on Gibbs Monolayer Formation by Biomolecules

Using glucose oxidase and salmon testis-derived DNA molecules, we sought to extend the recently proposed idea of interfacial adsorption denaturation. The surface pressure-time (π-t) isotherm Gibbs monolayer exhibited a rapid increase in pressure relatively prompt transition liquid condensed film. appearance this expansion phase occurred much earlier than that previously identified for lysozyme, trypsin, cytochrome C, luciferase. This experimental finding was linked number hydrophobic residues constituent unit, highest among these biomolecules. On other hand, molecules do not have such groups, or present positive on π-t curve. However, occurred, existence at air/water interface confirmed, even two-dimensional gas state. Furthermore, it confirmed an detected during formation mixed film biomolecules, forming stable monolayer. mimic behavior with matrix Langmuir monolayers. Moreover, clarified denaturation changed when pH dependence evaluated considering isoelectric point biomolecular group.